| 嗜热链球菌CGMCC 1.1864所产的一种新型细菌素ST9 |
| |
| 引用本文： | 李琳,贾士儒,谭之磊,杨洪江,高玉荣,范宝庆,王国良.嗜热链球菌CGMCC 1.1864所产的一种新型细菌素ST9[J].微生物学通报,2010,37(3):0349-0354. |
| |
| 作者姓名： | 李琳 贾士儒 谭之磊 杨洪江 高玉荣 范宝庆 王国良 |
| |
| 作者单位： | 工业微生物教育部重点实验室,天津科技大学,天津,300457 |
| |
| 基金项目： | 国家973计划项目(No. 2007CB714305); 国家863计划项目(No. 2006AA10Z347) |
| |
| 摘 要： | 本文利用琼脂扩散法测定嗜热链球菌(Streptocccus thermophilus) CGMCC 1.1864发酵上清液的抑菌效果, 结果表明此菌能够产生抑菌物质, 且在排除有机酸和过氧化氢的影响后上清液不但能抑制革兰氏阳性菌, 对革兰氏阴性菌也有抑制能力。此抑菌物质具有热稳定性, 于100°C处理
2 h及121°C处理20 min仍保留抑菌活性, 但若将其在100°C处理2 h的上清液立即置于?20°C保存, 其抑菌活性有较大损失。常温下(37°C), 该抑菌物质在pH 2.0?9.0范围内有很好的稳定性。发酵上清液经各种蛋白酶及?-淀粉酶处理后抑菌活性完全消失, 而对过氧化氢酶不敏感, 表明此抑菌物质为多肽, 属于细菌素, 本文初步将其命名为嗜热链球菌素ST9。由于ST9对其产生菌具有吸附作用, 选择pH吸附释放法对该嗜热链球菌素进行粗提, 然后经SephadexG-25凝胶层析柱除去杂蛋白, 最后冷冻干燥得纯品。通过Tricine-SDS-PAGE分析得到其分子量约为5.0 kD。
|
| 关 键 词： | 嗜热链球菌 细菌素 纯化 抑菌活性 抑菌谱 |
Thermophilin ST9, a New Bacteriocin Produced by Streptococcus thermophilus CGMCC 1.1864 |
| |
| LI Lin,JIA Shi-Ru,TAN Zhi-Lei,YANG Hong-Jiang,GAO Yu-Rong,FAN Bao-Qing and WANG Guo-Liang.Thermophilin ST9, a New Bacteriocin Produced by Streptococcus thermophilus CGMCC 1.1864[J].Microbiology,2010,37(3):0349-0354. |
| |
| Authors: | LI Lin JIA Shi-Ru TAN Zhi-Lei YANG Hong-Jiang GAO Yu-Rong FAN Bao-Qing and WANG Guo-Liang |
| |
| Institution: | Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin University of Science & Technology, Tianjin 300457, China;Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin University of Science & Technology, Tianjin 300457, China;Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin University of Science & Technology, Tianjin 300457, China;Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin University of Science & Technology, Tianjin 300457, China;Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin University of Science & Technology, Tianjin 300457, China;Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin University of Science & Technology, Tianjin 300457, China;Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin University of Science & Technology, Tianjin 300457, China |
| |
| Abstract: | In this study,antimicrobial activity of the culture supernatant of Streptococcus thermophilus CGMCC 1.1864 was investigated with the agar diffusion method.The subsequent experiments results indicated the antimicrobial activity of the supernatant were not due to the existence of hydrogen peroxide and organic acid in the solution.The antimicrobial spectrum assays showed that the supernatant could inhibit the growth of both Gram-negative bacteria and Gram-positive bacteria.Heat stability test was also performe... |
| |
| Keywords: | Streptococcus thermophilus Bacteriocin Purification Antimicrobial activity Antimicrobial spectrum |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《微生物学通报》浏览原始摘要信息 |
| 点击此处可从《微生物学通报》下载免费的PDF全文 |
|
