High-level expression and purification of mature HIV-1 protease in Escherichia coli under control of the araBAD promoter |
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Authors: | Alison Taylor David P Brown Sunil Kadam Mary Maus William E Kohlbrenner Debra Weigl Mary C Turon Leonard Katz |
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Institution: | (1) Corporate Molecular Biology, Abbott Laboratories, D93D AP9A/3, One Abbott Park Road, 60064 Abbott Park, IL, USA;(2) Anti-infective Research Division, Abbott Laboratories, D93D AP9A/3, One Abbott Park Road, 60064 Abbott Park, IL, USA;(3) Present address: Franklin College of Arts and Science, Department of Genetics, University of Georgia, 30602 Athens, GA, USA |
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Abstract: | Summary A 1.3-kb segment of Escherichia coli DNA containing the regulatory gene, araC, and the promoter of the araBAD operon was amplified by the polymerase chain reaction (PCR) and cloned into pUC18, resulting in plasmid pKB130 that produced the fragment of -galactosidase upon addition of L-arabinose (L-ara). A synthetic gene for human immunodeficiency virus (HIV)-1 preprotease was placed downstream of the araBAD promoter in pKB130 to create a translational fusion inducible by addition of L-ara. The fusion protein correctly autoprocessed in vivo to yield a mature 99-amino-acid HIV-1 protease, which was found predominantly in inclusion bodies. This material could be refolded to an active form, which was purified to homogeneity. A small fraction of the protease was expressed in vivo as a soluble active form, which allowed the monitoring of expression during fermentation by a rapid and simple whole cell assay employing an HIV-1 protease-specific fluorogenic substrate. |
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