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Electrophoresis of Derivatized Polyethylene Glycols: A Useful Method for Monitoring of Reactions on Soluble Polymeric Carrier
Authors:Jaroslav??ebestík,Petr?Niederhafner,Martin??afa?ík,Jan?Hlavá?ek  author-information"  >  author-information__contact u-icon-before"  >  mailto:honzah@uochb.cas.cz"   title="  honzah@uochb.cas.cz"   itemprop="  email"   data-track="  click"   data-track-action="  Email author"   data-track-label="  "  >Email author
Affiliation:(1) Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, 166 10 Flemingovo nám.2, 166 10 Prague 6, Czech Republic
Abstract:A useful method for monitoring of peptide synthesis on polyethylene glycol (PEG) was developed and is described in this paper. The course of reactions such as loading, acylation, detachment and others where a charge of reactant and product differ, can be easily determined. The loading of the first amino acid is quantified with errors fitting well to those from other known analytical methods.
Keywords:PEG  mPEG  cobalt staining  electrophoresis  colorimetry
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