Effects of hypochlorite disinfection on the response of barley aleurone layers to gibberellic acid |
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Authors: | J. Stephen Goudey Forrest L. Tittle Mary S. Spencer |
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Affiliation: | Dept of Plant Science, Univ. of Alberta, Edmonton, Alberta, Canada, T6G 2P5. |
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Abstract: | Although the use of hypochlorite to disinfect seeds is widespread, the effects on tissues isolated from them have been largely ignored. Disinfection of barley ( Hordeum vulgare L. cv. Himalaya) half-seeds with hypochlorite solutions of ≥1.0% (w/v) available chlorine caused the pericarp to separate from the underlying tissues. Aleurone layers isolated from these grains had lower rates of oxygen consumption and released significantly less protein, PO43− Mg2+, K+ and amylase (EC 3.2.1.1) into the medium in response to gibberellic acid (GA3) than layers isolated from grains disinfected with a 0.1% hypochlorite solution. Disinfection with 1.0% hypochlorite also quantitatively altered the spectrum of proteins released into the incubation medium by the layers in response to GA3. |
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Keywords: | Amylase isozymes ion release oxygen consumption |
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