Characterization of chromosome and plasmid transformation in Bacillus subtilis using gently lysed protoplasts |
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| Authors: | T Akamatsu J Sekiguchi |
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| Institution: | (1) Department of Applied Microbiology and Technology, Kumamoto Institute of Technology, Ikeda 4-22-1, 860 Kumamoto, Japan;(2) Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, 3-15-1 Tokida, 386 Ueda-shi, Nagano, Japan |
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| Abstract: | Competent cells of Bacillus subtilis were transformed with DNA from gently lysed protoplasts. Significant linkages among markers separated by distances of approximately 2.3% of the total chromosome were found, which have not been detected for conventional transformation. In comparison to previous reports, enhanced plasmid transformation was observed 4.0×107 transformants per  g DNA (one transformant per 5×104 molecules added)], when competent cells were transformed with DNA from lysed protoplasts harboring pUB110. |
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| Keywords: | Protoplast Plasmid Transformation Bacillus subtilis Linkage |
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