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Structural analysis of conjugated linoleic acid produced by Lactobacillus plantarum,and factors affecting isomer production
Authors:Kishino Shigenobu  Ogawa Jun  Ando Akinori  Iwashita Takashi  Fujita Tsuyoshi  Kawashima Hiroshi  Shimizu Sakayu
Affiliation:Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
Abstract:An isomer of the conjugated linoleic acid (CLA) produced from linoleic acid by Lactobacillus plantarum was identified as cis-9,trans-11-octadecadienoic acid by proton nuclear magnetic resonance spectroscopy. Together with earlier results, we concluded that the bacterium produces two CLA isomers, cis-9,trans-11- and trans-9,trans-11-octadecadienoic acid from linoleic acid. The addition of L-serine, glucose, AgNO3, or NaCl to the reaction mixture reduced production of the latter.
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