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Measurement of DNA with an automatic spectrophotometer |
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| Authors: | T C Smeaton R D Eichner |
| Institution: | 1. Department of Physiological Chemistry, University of Wisconsin Medical School, Madison, Wisconsin 53706, USA;2. Department of Pediatrics, University of Wisconsin Medical School, Madison, Wisconsin 53706, USA;3. Department of Molecular Biology, Albert Einstein College of Medicine, Brons, New York 10461 USA |
| Abstract: | A method that uses an automated spectrophotometer to read microtiter plates in a modification of the diphenylamine method of DNA determination in tissue extracts, is presented. The micromethod saves technician time, and reduces exposure to acetic acid fumes. The reproducibility of the method is superior to the manual method. Fewer pipetting steps are required, and the method is suited to large batches of samples. |
| Keywords: | calmodulin methylation affinity chromatography post-translational modification radiometric enzyme assay To whom correspondence and reprint requests should be addressed: 655 Waisman Center University of Wisconsin Madison Wisc 53706 |
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