| 表达HPV16E6和E7蛋白的非复制型重组痘苗病毒的构建 |
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| 引用本文： | 骆卫锋,韩立群,任皎,田厚文,陆振华,谷淑燕,阮力.表达HPV16E6和E7蛋白的非复制型重组痘苗病毒的构建[J].病毒学报,2002,18(3):217-221. |
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| 作者姓名： | 骆卫锋 韩立群 任皎 田厚文 陆振华 谷淑燕 阮力 |
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| 作者单位： | 中国疾病预防控制中心,病毒病预防控制所,北京,100052 |
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| 基金项目： | 欧盟国际合作项目;ERBICI18-CT97-0234; |
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| 摘 要： | 为研制HPV16的治疗性疫苗,首先将表达质粒pJSA1175与非复制型痘苗病毒NTVJTK+进行同源重组,构建了痘苗重组病毒NTVJLac.再将表达质粒pJSDME6E7R与NTVJLac进行同源重组,构建了表达HPV16 E6和E7蛋白的非复制型重组痘苗病毒NTVJmE6E7,并对获得的重组病毒进行了鉴定.Southern杂交显示,重组痘苗病毒NTVJmE6E7基因组中有E6和E7基因插入.该重组病毒在人源细胞中不复制.Western blot显示,重组病毒在人源TK-143细胞中能表达E6和E7蛋白.非复制型重组痘苗病毒NTVJmE6E7可作为HPV16相关肿瘤及其癌前病变免疫治疗的实验性疫苗株.
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| 关 键 词： | 乳头瘤病毒 宫颈肿瘤 痘苗病毒 疫苗 免疫治疗 |
| 文章编号： | 1000-8721(2002)03-0217-05 |
Construction of Non-replicating Recombinant Vaccinia Virus Expressing HPV16 E6 and E7 Proteins |
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| LUO Wei feng,HAN Li qun,REN Jiao,TIAN Hou wen,LU Zhen hua,GU Shu yan,RUAN Li.Construction of Non-replicating Recombinant Vaccinia Virus Expressing HPV16 E6 and E7 Proteins[J].Chinese Journal of Virology,2002,18(3):217-221. |
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| Authors: | LUO Wei feng HAN Li qun REN Jiao TIAN Hou wen LU Zhen hua GU Shu yan RUAN Li |
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| Abstract: | To develop antigen-specific theraperutic vaccine for HPV16-associated tumors and their precancerous transformations,a non-replicating recombinant vaccinia virus NTVJLac was constructed through the homologous recombination between vector pJSA1175 and NTVJTK + Then non-replicating recombinant vaccinia virus NTVJmE6E7 was constructed by replacing the LacZ gene from NTVJLac with mE6 and mE7-1 genes of pJSDME6E7R through homologous recombination Southern blot showed mE6 and mE7-1 genes had been inserted in recombinant NTVJmE6E7 NTVJmE6E7 could not replicate in human cells Western blot showed NTVJmE6E7 could express mE6 and mE7 proteins in TK -143 cells NTVJmE6E7 could be taken as a candidate of therapeutic vaccine for HPV-associated tumors and their precancerous transformations |
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| Keywords: | papillomavirus cervical neoplasm vaccinia virus vaccine immunotherapy |
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