SEMIAUTOMATED FLUORIMETRIC DETERMINATION OF TYROSINE IN RAT BRAIN AND HUMAN PLASMA |
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| Authors: | Gwyneth Evans Margot K Hartmann Liliana Wood M K Gaitonde |
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| Institution: | Department of Biochemistry and Department of Psychiatry, London SW17 ORE, England;Department of Biochemistry St. George's Hospital Medical School. Cranmer Terrace, London SW17 ORE, England;Department of Biochemistry. Royal Dental School, Cranmer Terrace. London SW17 ORE. England |
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| Abstract: | Abstract— The evidence is presented to show that the fiuorimetric method of W aalkes & U denfriend (1957) cannot be used directly for the determination of free tyrosine in acid deproteinized extracts of rat brain, owing to the reaction of peptide bound tyrosine in some proteins present in the acid extracts. A method is given to obtain a fraction containing free tyrosine in the acid extracts of rat brain and to determine its tyrosine content fluorimetrically by autoanalysis. An autoanalysis method is also given for the fiuorimetric determination of free tyrosine directly in 50 trichloroacetic acid extracts of the plasma. |
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