Demonstration by immunoblotting of heterogeneity in the autoantibody response directed against fat cells in Graves' disease |
| |
| Authors: | A P Weetman T B Nutman J R Baker K D Burman |
| |
| Affiliation: | 1. Department of Medicine, Royal Postgraduate Medical School, Du Cane Road, London W12 OHS, England;2. Laboratory of Parasitic Diseases, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 USA;3. Endocrine and Transplantation Services, Walter Reed Army Medical Center, Washington, DC 20307, USA |
| |
| Abstract: | Guinea pig fat cell membranes (FCM) have been widely used in preference to thyroid membranes as a source of TSH receptors to investigate TSH receptor antibodies in Graves' disease, because FCM are ostensibly free of other thyroid antigens. However, by FCM immunoblotting we have found: 8 of 10 normal sera bound to determinants at 38 and 190 kDa; 17 other determinants were recognised by 60% of Graves' or Hashimoto sera and by 20% of normal sera; three determinants at 65-90 kDa were recognised by 5 of 13 Graves' but by none of the normal or Hashimoto sera; and none of the determinants recognised appeared to be related to the TSH receptor. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
