Identification of legumin-like proteins in wheat |
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| Authors: | Nagendra K. Singh Kenneth W. Shepherd Peter Langridge L. Clem Gruen John H. Skerritt Colin W. Wrigley |
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| Affiliation: | (1) Agronomy Department, Waite Agricultural Research Institute, 5064 Glen Osmond, South Australia, Australia;(2) Agricultural Biochemistry Department, Waite Agricultural Research Institute, 5064 Glen Osmond, South Australia, Australia;(3) CSIRO Division of Biotechnology, 3052 Parkville, Victoria, Australia;(4) (Wheat Research Unit), CSIRO Division of Plant Industry, 2113 North Ryde, N.S.W., Australia |
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| Abstract: | We have obtained several amino acid sequences from purified polypeptides of a wheat endosperm storage globulin previously described as  triplet protein. The amino acid sequence data supported by immunochemical analysis using anti-oat 12S globulin antibodies, provide definitive evidence that the triplet protein is homologous to pea legumin and related seed storage proteins of oats, rice and several dicotyledonous species. Thus, it is now proposed that the triplet protein of wheat be renamed triticin. The oat globulin antibodies also cross-reacted strongly with the high-molecular-weight (HMW) glutenin subunits which have been implicated in bread-making quality. |
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| Keywords: | wheat triplet protein (triticin) legumin amino acid sequence homology |
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