Extracts of plasma and serum toxic for mammalian cells cultured in vitro |
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| Authors: | C De Luca C Carruthers G L Tritsch |
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| Affiliation: | 1. Department of Female Pelvic Medicine and Reconstructive Surgery, Montefiore Health System, New York, New York, United States;2. Department of Obstetrics and Gynecology, Stamford Hospital, Stamford, Connecticut, United States;3. Department of Research and Discovery, Stamford Hospital, Stamford, Connecticut, United States;4. Department of Urogynecology and Reconstructive Surgery, Stamford Hospital, Stamford, Connecticut, United States;1. College of Sciences and Mathematics and Molecular Biosciences Program, Arkansas State University, Jonesboro, Arkansas, United States;2. Department of Biological Sciences, Arkansas State University, Jonesboro, Arkansas, United States;3. Audubon Delta, National Audubon Society, Baton Rouge, Louisiana, United States;4. United States Department of Agriculture, Natural Resources Conservation Service, Addis, Louisiana, United States;1. College of Finance and Information, Ningbo University of Finance and Economics, Ningbo 315175, PR China;2. School of Physics and Astronomy, Yunnan University, Kunming 650091, PR China |
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| Abstract: | - 1.1. The participation of two components in the cytotoxic activity of extracts of certain preparations of bovine plasma alpha-globulins (Cohn Fraction IV-1) is described. One is a heat-labile, non-dialyzable material assumed to be a protein. The other, lipid in nature, can be extracted from whole and lyophilized calf serum as well as from bovine alpha-globulins.
- 2.2. The role of the protein has not been established; it apparently binds the lipid. When it is present, it must be in the native state for the expression of the cytotoxic property.
- 3.3. Lipid extracts are clearly toxic per se. Even after denaturation of the protein and apparent loss of cytotoxic activity, active lipids can be released with organic solvents. They have been separated and identified by column and thin layer chromatography. Active compounds taken from thin layer chromatograms appear to be fatty acids.
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