|
|||||
|
|
Aluminum ions stimulate the oxidizability of low density lipoprotein by Fe2+: implication in hemodialysis mediated atherogenic LDL modification |
|
| Authors: | Kapiotis Stylianos Hermann Marcela Exner Markus Sturm Brigitte N Scheiber-Mojdehkar Barbara Goldenberg Hans Kopp Stefan Chiba Peter Gmeiner Bernhard M K |
| Affiliation: | a Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Vienna, Vienna, Austriab The Central Laboratory, Neunkirchen, Austriac Department of Medical Biochemistry, Max F. Perutz Laboratories, University Departments at the Vienna Biocenter, Medical University of Vienna, Austriad Centre of Physiology and Pathophysiology, Institute of Medical Chemistry, Medical University of Vienna, Vienna, Austria |
| Abstract: | Objective: Al3+ stimulates Fe2+ induced lipid oxidation in liposomal and cellular systems. Low-density lipoprotein (LDL) oxidation may render the particle atherogenic. As elevated levels of Al3+ and increased lipid oxidation of LDL are found in sera of hemodialysis patients, we investigated the influence of Al3+ on LDL oxidation. Materials and methods: Using different LDL modifying systems (Fe2+, Cu2+, free radical generating compounds, human endothelial cells, hemin/H2O2 and HOCl), the influence of Al3+ on LDL lipid and apoprotein alteration was investigated by altered electrophoretic mobility, lipid hydroperoxide-, conjugated diene- and TBARS formation. Results: Al3+ could stimulate the oxidizability of LDL by Fe2+, but not in the other systems tested. Al3+ and Fe2+ were found to bind to LDL and Al3+could compete with Fe2+ binding to the lipoprotein. Fluorescence polarization data indicated that Al3+ does not affect the phospholipid compartment of LDL. Conclusions:The results indicate that increased LDL oxidation by Fe2+ in presence of Al3+ might be due to blockage of Fe2+ binding sites on LDL making more free Fe2+ available for lipid oxidation. |
| Keywords: | LDL oxidation aluminum ions iron ions hemodialysis atherosclerosis |
| 本文献已被 InformaWorld PubMed 等数据库收录！ | |