Sequence of histidyl tRNA,present as a chloroplast insert in mtDNA ofZea mays |
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| Authors: | Keith P Iams Joyce E Heckman John H Sinclair |
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| Institution: | (1) Present address: ILRAD, P.O. Box 30709, Nairobi, Kenya;(2) Department of Biology, Indiana University, 47405 Bloomington, IN, USA;(3) Department of Chemistry, Indiana University, 47405 Bloomington, IN, USA |
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| Abstract: | Unfractionated tRNA, isolated from maize mitochondria, has been specifically labeled at the -CCA end and used to recover a
tRNA gene-bearing fragment from a clone bank of maize mitochondrial DNA. This gene has been mapped, sequenced and found to
carry the anticodon for histidine. The sequence of the gene and that of bases in its near vicinity are identical to maize
chloroplast tRNAHis, although sequences more distant on the fragment are not homologous with cpDNA. The junction of the cpDNA insert has been
sequenced. |
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| Keywords: | chloroplast DNA maize mitochondria sequence tRNAHis |
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