Isolation of a polygalacturonase-inhibiting protein (PGIP) from wheat |
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Authors: | Kemp Gabré Bergmann Carl W Clay Ron Van der Westhuizen Amie J Pretorius Zacharias A |
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Institution: | Department of Plant Sciences, University of the Free State, PO Box 339, Bloemfontein 9300, South Africa. gabre@ccrc.uga.edu |
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Abstract: | Evidence for the presence of a polygalacturonase-inhibiting protein (PGIP) from a monocotyledonous cereal is presented. A 40.3-kDa PGIP that was closely associated with the cell wall was acetone-extracted and purified from wheat (Triticum aestivum L.) leaves and stems. Wheat PGIP exhibited a highly selective inhibitory activity against endopolygalacturonase (EPG) from various fungi. Of nine EPG tested, wheat PGIP only inhibited EPG from Cochliobolus sativus, a pathogen of the tribe Poaceae. A short N-terminal amino acid sequence of wheat PGIP shows no similarity to any other characterized PGIP. |
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