Impairment of survival signaling and efferocytosis in TRPC3-deficient macrophages |
| |
Authors: | Tano Jean-Yves Smedlund Kathryn Lee Robert Abramowitz Joel Birnbaumer Lutz Vazquez Guillermo |
| |
Affiliation: | aDepartment of Physiology and Pharmacology and the Center for Diabetes and Endocrine Research, University of Toledo College of Medicine, Health Science Campus, 3000 Arlington Av, Toledo, OH 43614, USA;bLaboratory of Membrane Signaling, Department of Signal Transduction, National Institute of Environmental Health Science, Research Triangle Park, NC 23709, USA |
| |
Abstract: | We have recently shown that in macrophages proper operation of the survival pathways phosphatidylinositol-3-kinase (PI3K)/AKT and nuclear factor kappa B (NFkB) has an obligatory requirement for constitutive, non-regulated Ca2+ influx. In the present work we examined if Transient Receptor Potential Canonical 3 (TRPC3), a member of the TRPC family of Ca2+-permeable cation channels, contributes to the constitutive Ca2+ influx that supports macrophage survival. We used bone marrow-derived macrophages obtained from TRPC3−/− mice to determine the activation status of survival signaling pathways, apoptosis and their efferocytic properties. Treatment of TRPC3+/+ macrophages with the pro-apoptotic cytokine TNFα induced time-dependent phosphorylation of IκBα, AKT and BAD, and this was drastically reduced in TRPC3−/− macrophages. Compared to TRPC3+/+ cells TRPC3−/− macrophages exhibited reduced constitutive cation influx, increased apoptosis and impaired efferocytosis. The present findings suggest that macrophage TRPC3, presumably through its constitutive function, contributes to survival signaling and efferocytic properties. |
| |
Keywords: | Abbreviations: CAM, calmodulin CAMKII, calmodulin dependent kinase II PI3K, phosphatidylinositol-3-kinase NFkB, nuclear factor kappa B TNFα, tumor necrosis factor alpha TRPC, Transient Receptor Potential Canonical |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|