Overexpression of HDAC1 induces cellular senescence by Sp1/PP2A/pRb pathway |
| |
| Authors: | Chuang Jian-Ying Hung Jan-Jong |
| |
| Affiliation: | aDepartment of Operative Dentistry and Periodontology, University Hospital Regensburg, Regensburg, Germany;bInstitute of Complex Systems, ICS7: Biomechanics, Forschungszentrum Jülich, Jülich, Germany;cDepartment of Gynecology and Obstetrics, University of Regensburg, Germany;dDepartment of Oral and Maxillofacial Surgery, University Hospital Regensburg, Regensburg, Germany |
| |
| Abstract: | The differentiation of stem cells can be directed by the grade of stiffness of the developed tissue cells. For example a rigid extracellular matrix supports the osteogenic differentiation in bone marrow derived mesenchymal stem cells (MSCs). However, less is known about the relation of extracellular matrix stiffness and cell differentiation of ectomesenchymal dental precursor cells. Our study examined for the first time the influence of the surface stiffness on the proliferation and osteogenic differentiation of human dental follicle cells (DFCs). Cell proliferation of DFCs was only slightly decreased on cell culture surfaces with a bone-like stiffness. The osteogenic differentiation in DFCs could only be initiated with a dexamethasone based differentiation medium after using varying stiffness. Here, the softest surface improved the induction of osteogenic differentiation in comparison to that with the highest stiffness. In conclusion, different to bone marrow derived MSCs, soft ECMs have a superior capacity to support the osteogenic differentiation of DFCs. |
| |
| Keywords: | Dental follicle cells PDMS Differentiation Proliferation |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
