Sphingolipid long chain base phosphates can regulate apoptotic-like programmed cell death in plants |
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Authors: | Alden Keith P Dhondt-Cordelier Sandrine McDonald Kerrie L Reape Theresa J Ng Carl K-Y McCabe Paul F Leaver Christopher J |
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Institution: | aSchool of Biology and Environmental Science, University College Dublin, Dublin 4, Ireland;bDepartment of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3PS, UK |
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Abstract: | Sphingolipids are ubiquitous components of eukaryotic cells and sphingolipid metabolites, such as the long chain base phosphate (LCB-P), sphingosine 1 phosphate (S1P) and ceramide (Cer) are important regulators of apoptosis in animal cells. This study evaluated the role of LCB-Ps in regulating apoptotic-like programmed cell death (AL-PCD) in plant cells using commercially available S1P as a tool. Arabidopsis cell cultures were exposed to a diverse array of cell death-inducing treatments (including Cer) in the presence of S1P. Rates of AL-PCD and cell survival were recorded using vital stains and morphological markers of AL-PCD. Internal LCB-P levels were altered in suspension cultured cells using inhibitors of sphingosine kinase and changes in rates of death in response to heat stress were evaluated. S1P reduced AL-PCD and promoted cell survival in cells subjected to a range of stresses. Treatments with inhibitors of sphingosine kinase lowered the temperature which induced maximal AL-PCD in cell cultures. The data supports the existence of a sphingolipid rheostat involved in controlling cell fate in Arabidopsis cells and that sphingolipid regulation of cell death may be a shared feature of both animal apoptosis and plant AL-PCD. |
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Keywords: | Apoptotic-like programmed cell death Arabidopsis Cell cultures Sphingolipids |
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