SUMO1 attenuates stress-induced ROS generation by inhibiting NADPH oxidase 2 |
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Authors: | Kim Hyun Jung Yun Jiwon Lee Jiyoung Hong Hyunkyung Jeong Jaeho Kim Eunhee Bae Yun Soo Lee Kong-Joo |
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Affiliation: | aThe Center for Cell Signaling & Drug Discovery Research, College of Pharmacy, Division of Life and Pharmaceutical Sciences, Department of Bioinspired Science, Ewha Womans University, Seoul 120-750, South Korea;bDivision of Life Science, Chungnam National University, Daejeon 305-764, South Korea |
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Abstract: | Small ubiquitin-like modifier 1 (SUMO1) is a member of the superfamily of ubiquitin-like proteins. Despite its structural similarity with ubiquitin, SUMO1 does not seem to play any role in protein degradation and its precise biological function is poorly understood. During our studies on heat-shock responses, we found that heat-shock stress increased SUMO1 conjugation in a dose-dependent manner. Intriguingly, SUMO1 conjugation resulted in decrease of intracellular ROS generation and protection cells from death under heat-shock stress. We showed that NADPH oxidase 2 (NOX2) is a target protein of sumoylation by SUMO1 using immunoprecipitation and is colocalized with SUMO1 at plasma membrane. Additionally, we demonstrated that the attenuation in intracellular ROS generation resulted from inhibition of NADPH oxidase complex (NOX) activity. These results suggested that SUMO1 plays an important role in modulation of NOX activity required for ROS generation. |
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Keywords: | SUMO1 Heat-shock Reactive oxygen species (ROS) NADPH oxidase complex (NOX) |
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