The anti-inflammatory effects of soluble epoxide hydrolase inhibitors are independent of leukocyte recruitment |
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Authors: | Davis Benjamin B Liu Jun-Yan Tancredi Daniel J Wang Lei Simon Scott I Hammock Bruce D Pinkerton Kent E |
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Affiliation: | aCenter for Health and the Environment, University of California, Davis, CA 95616, United States;bDepartment of Entomology, University of California, Davis, CA 95616, United States;cDepartment of Pediatrics, University of California, Davis, CA 95616, United States;dDepartment of Biomedical Engineering, University of California, Davis, CA 95616, United States |
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Abstract: | Excess leukocyte recruitment to the lung plays a central role in the development or exacerbation of several lung inflammatory diseases including chronic obstructive pulmonary disease. Epoxyeicosatrienoic acids (EETs) are cytochrome P-450 metabolites of arachidonic acid reported to have multiple biological functions, including blocking of leukocyte recruitment to inflamed endothelium in cell culture through reduction of adhesion molecule expression. Inhibition of the EET regulatory enzyme, soluble epoxide hydrolase (sEH) also has been reported to have anti-inflammatory effects in vivo including reduced leukocyte recruitment to the lung. We tested the hypothesis that the in vivo anti-inflammatory effects of sEH inhibitors act through the same mechanisms as the in vitro anti-inflammatory effects of EETs in a rat model of acute inflammation following exposure to tobacco smoke. Contrary to previously published data, we found that sEH inhibition did not reduce tobacco smoke-induced leukocyte recruitment to the lung. Furthermore, sEH inhibition did not reduce tobacco smoke-induced adhesion molecule expression in the lung vasculature. Similarly, concentrations of EETs greater than or equal to their reported effective dose did not reduce TNFα induced expression of the adhesion molecules. These results suggest that the anti-inflammatory effects of sEH inhibitors are independent of leukocyte recruitment and EETs do not reduce the adhesion molecules responsible for leukocyte recruitment in vitro. This demonstrates that the widely held belief that sEH inhibition prevents leukocyte recruitment via EET prevention of adhesion molecule expression is not consistently reproducible. |
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Keywords: | Abbreviations: sEH, soluble epoxide hydrolase EETs, epoxyeicosatrienoic acids SH, spontaneously hypertensive TSP, total suspended particulates BAL, bronchoalveolar lavage BALF, bronchoalveolar lavage fluid HLMVEC, human lung microvascular endothelial cells HUVEC, human umbilical vein endothelial cells sEHI, sEH inhibition CI, confidence intervals TS, tobacco smoke LPS, lipopolysaccharide LTX, leukotoxins StARD1, acute neurosteroid producing gene |
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