| 气质联用定量分析放线菌全细胞单糖方法的研究* |
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| 引用本文： | 陈文峰, 蒋凌月, 徐丽华, 姜成林,.气质联用定量分析放线菌全细胞单糖方法的研究*[J].微生物学通报,2000,27(6):416-420. |
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| 作者姓名： | 陈文峰 蒋凌月 徐丽华 姜成林 |
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| 作者单位： | 云南大学微生物研究所,昆明,650091 |
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| 基金项目： | 云南省科委国际合作项目 |
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| 摘 要： | 采用气相色谱－质谱联用仪分析了近百株放线菌的全细胞水解液单糖。放线菌全细胞糖经酸水解、氢化还原和乙酯化生成糖醇乙酸酯衍生物通过气相色谱－质谱仪定性、定量分析。鼠李糖、核糖、阿拉伯糖、木糖、马杜拉糖、甘露糖、葡萄糖和半乳糖的糖醇乙酸酯衍生物在这种条件下得到了良好地分离。计算各糖的峰面积之比确定它们的相对百分含量。
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| 关 键 词： | 气相色谱－质谱联用 定量分析 放线菌 全细胞单糖 |
| 文章编号： | 0253-2654(2000)06-0416-05 |
| 修稿时间： | 1999年7月19日 |
STUDIES ON QUANTITATIVE ANANLYSIS OF WHOLE-CELL SUGARSIN ACTINOMYCETES BY GAS CHROMATOGRAPHY-MASS SPECTRUM |
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| CHEN Wen-Feng,JIANG Ling-Yue,XU Li-Hua,JIANG Cheng-Lin.STUDIES ON QUANTITATIVE ANANLYSIS OF WHOLE-CELL SUGARSIN ACTINOMYCETES BY GAS CHROMATOGRAPHY-MASS SPECTRUM[J].Microbiology,2000,27(6):416-420. |
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| Authors: | CHEN Wen-Feng JIANG Ling-Yue XU Li-Hua JIANG Cheng-Lin |
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| Abstract: | Rapidly and accurately quantitative analysis of whole-cell sugars in some strains of actinomycetes by GC-MS was performed in this paper. After the polysaccharides were hydrolyzed, deoxidized and esterified , the alditol acetate derivates was analyzed by GC-MS. The derivates of rhamnose, ribose, arabinose , xylose, madurose, mannose, glucose, galactose were separated commendably under this condition. The ratio of the peak area was calculated to get the relative percentage of the various sugars. |
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| Keywords: | GC-MS Quantitative analysis Whole-cell sugars of actinomycete| |
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