Cytogenetic study of <Emphasis Type="Italic">Ascaris</Emphasis> trypsin inhibitor in cultured human lymphocytes with metabolic activation |
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| Authors: | Joanna Blaszkowska Wanda Bratkowska Dobroslawa Lopaczynska Tomasz Ferenc |
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| Institution: | (1) Department of Biology and Medical Parasitology, Medical University of Lodz, Pl. Hallera 1, 90-647 Lodz, Poland;(2) Department of Biology and Genetics, Chair of Biology and Medical Genetics, Medical University of Lodz, Pl. Hallera 1, 90-647 Lodz, Poland |
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| Abstract: | The trypsin inhibitor (ATI) isolated from gastrointestinal nematode Ascaris suum was tested in vitro for induction of chromosome aberrations and sister chromatid exchanges (SCE). Genotoxicity assessment of purified ATI was
carried out on metaphase plates received from peripheral blood lymphocyte macroculture (48 h test of structural chromosome
aberrations and 72 h test of SCE) with exogenous metabolic activation. ATI was tested in dose of 25, 50 and 100 μg per ml
of culture. Kinetics of cell divisions were determined by the replication index (RI). The mitotic index (MI) was expressed
as a number of metaphases per 1000 nuclei analysed. Analysis of chromosome aberrations showed that higher doses of ATI (50
and 100 μg/ml) significantly increased the frequency of chromosome aberrations (mainly of chromatid gaps and breaks) compared
to the negative control. All concentrations of ATI caused a statistically significant reduction in the MI and RI. In comparison
with the negative control, a significant increase in the SCE frequency was observed in all applied doses of ATI. Thus, in
the presence of S9 activation, the Ascaris trypsin inhibitor showed potential clastogenic activity and inhibition of the dynamics of lymphocyte divisions. |
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| Keywords: | Ascaris suum chromosome aberrations mitotic index replication index sister chromatid exchanges trypsin inhibitor |
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