Simple, rapid, and quantitative release of periplasmic proteins by chloroform. |
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Authors: | G F Ames C Prody S Kustu |
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Abstract: | We introduce a method by which periplasmic proteins can be released rapidly, simply, and quantitatively by treating cells with chloroform. All the amino acid-binding proteins tested maintained their activity during chloroform treatment. This method makes practical the analysis of the periplasmic protein complement of a large number of strains. |
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