Modification of photosynthetic regulation in tomato overexpressing glutathione peroxidase |
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Authors: | Herbette Stephane Menn Aline Le Rousselle Patrick Ameglio Thierry Faltin Zehava Branlard Gérard Eshdat Yuval Julien Jean-Louis Drevet Joël R Roeckel-Drevet Patricia |
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Institution: | UMR 547-PIAF, INRA/Université Blaise Pascal, 63177 Aubière, France. |
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Abstract: | To investigate the function of glutathione peroxidase (GPX) in plants, we produced transgenic tomato plants overexpressing an eukaryotic selenium-independent GPX (GPX5). We show here that total GPX activity was increased by 50% in transgenic plants, when compared to control plants transformed with the binary vector without the insert (PZP111). A preliminary two-dimensional electrophoretic protein analysis of the GPX overexpressing plants showed notably a decrease in the accumulation of proteins identified as rubisco small subunit 1 and fructose-1,6-bisphosphate aldolase, two proteins involved in photosynthesis. These observations, together with the fact that in standard culture conditions, GPX-overexpressing plants were not phenotypically distinct from control plants prompted us to challenge the plants with a chilling treatment that is known to affect photosynthesis activity. We found that upon chilling treatment with low light level, photosynthesis was not affected in GPX-overexpressing plants while it was in control plants, as revealed by chlorophyll fluorescence parameters and fructose-1,6-biphosphatase activity. These results suggest that overexpression of a selenium-independent GPX in tomato plants modifies specifically gene expression and leads to modifications of photosynthetic regulation processes. |
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