Requirement of protein factors and ATP for the disassembly of the spliceosome after mRNA splicing reaction. |
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| Authors: | H Sawa and Y Shimura |
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| Institution: | Department of Biophysics, Faculty of Science, Kyoto University, Japan. |
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| Abstract: | Pre-mRNA splicing reaction occurs in a large ribonucleoprotein complex called the spliceosome. After the splicing reaction, the spliceosome is disassembled to release the splicing products including spliced mRNA. Here we show that protein factors in a HeLa nuclear extract or a DEAE-cellulose fraction as well as ATP are required to release the splicing products form the spliceosome in which the splicing reaction has already completed. |
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