Purification and N-Terminal Amino Acid Sequence of Dextranicin 24, a Bacteriocin of Leuconostoc sp. |
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| Authors: | Anne-Marie Revol-Junelles Gérard Lefebvre |
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| Affiliation: | (1) Laboratoire de Microbiologie Industrielle et Alimentaire, Ecole Nationale Supérieure d'Agronomie et des Industries Alimentaires, Institut National Polytechnique de Lorraine, 2 avenue de la Forêt de Haye, 54500 Vandoeuvre lès Nancy, France , FR |
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| Abstract: | Leuconostoc mesenteroides subsp. dextranicum strain J24 synthesized a bacteriocin named Dextranicin 24 (Dex-24), which inhibited only other Leuconostoc sp. strains. It was purified by a two-step procedure from the fraction of the bacteriocin bound to the producer cells at the end of the growth: desorption form the cells at acidic pH, followed by reserve phase HPLC. The N-terminal sequence of Dex-24 was the following: NH2− K G V L G W L S M A S S A L T G P Q Q . . . Received: 26 January 1996 / Accepted: 28 March 1996 |
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