Relationships in hydrogen metabolism between hydrogenase and nitrogenase in phototrophic bacteria. |
| |
Authors: | I N Gogotov |
| |
Institution: | Institute of Photosynthesis, USSR Academy of Sciences, Pushchino, Moscow Region, 142292 (USSR) |
| |
Abstract: | Purple bacteria Rhodospirillum rubrum and Thiocapsa roseopersicina form two enzymes, hydrogenase and nitrogenase, which participate in hydrogen metabolism. H2 photoproduction in these bacteria is associated mainly or completely with the action of nitrogenase. The soluble and membrane-bound hydrogenases of T. roseopersicina have similar physicochemical properties (mol. weight, subunit composition, N-terminal amino acids, Fe2+ and S2- content, pl. Eo'). In comparison with other hydrogenases the enzyme from R. rubrum and T. roseopersicina evolve H2 with high rate from reduced cytochrome c3, but not from ferredoxins. H2 production and N2 fixation take place in the presence of NAD(P)H. NADP-reductase, ferredoxin and cytochrome c3 participate in this reaction. Possible relationships between hydrogenase-nitrogenase in the metabolism of molecular hydrogen are discussed. |
| |
Keywords: | MY methyl viologen Fd ferredoxin |
本文献已被 ScienceDirect 等数据库收录! |
|