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| 鸭胚胎干细胞分离与鉴定 | |
| 引用本文： | 高健潜,张春红,王丙云,陈金顶. 鸭胚胎干细胞分离与鉴定[J]. 生物技术通报, 2010, 0(1) |
| 作者姓名： | 高健潜 张春红 王丙云 陈金顶 |
| 作者单位： | 1. 佛山科学技术学院动物医学系,佛山,528231;华南农业大学兽医学院,广州,510642 2. 华南农业大学兽医学院,广州,510642 3. 佛山科学技术学院动物医学系,佛山,528231 |
| 摘 要： | 采用药勺法分离仙湖3号肉鸭鸭胚的胚盘细胞,并以鸭胚成纤维细胞作为饲养层,培养鸭胚胎干细胞。在此基础上,通过对体外培养的鸭胚胎干细胞形态观察,碱性磷酸酶染色(AKP)以及胚胎阶段表面特异性抗原(SSEA-1)免疫组化等方法,分离与鉴定鸭胚胎干细胞。结果表明传至第3代的鸭胚胎干细胞经AKP和SSEA-1鉴定均为阳性,AKP染色为深蓝色,SSEA-1染色呈绿色荧光,表明培养至第3代的鸭胚胎干细胞仍保持干细胞未分化特性,具有胚胎干细胞的特征。结果提示本试验分离、培养的鸭胚盘细胞为鸭胚胎干细胞。 |
| 关 键 词： | 鸭 胚胎干细胞 分离 鉴定 |
Isolation and Identification of Ducks Embryonic Stem Cells in Vitro |
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| Gao Jianqian,Zhang Chunhong,Wang Bingyun,Chen Jinding. Isolation and Identification of Ducks Embryonic Stem Cells in Vitro[J]. Biotechnology Bulletin, 2010, 0(1) | |
| Authors: | Gao Jianqian Zhang Chunhong Wang Bingyun Chen Jinding |
| Affiliation: | Gao Jianqian1,2 Zhang Chunhong2 Wang Bingyun1 Chen Jinding2(1Faculty of Veterinary Medicine,Foshan University,Foshan 528231,2College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642) |
| Abstract: | In this study,BCs were isolated from hatching egg of No.3 Xianhu Ducks by spoon methods.ESCs were cultured on feeder layers of duck embryonic fibroblasts(DEF)in primary culture.The ESCs were identified by morphology,histochemical methods including AKP and SSEA-1.The results indicated that the 3rd passage ducks ESCs on feeder layers of DEF were positive to AKP and SSEA-1.It seemed that the 3rd passage ducks ESCs were undifferentiated characteristics under these culture system and maintained characters of ste... |
| Keywords: | Ducks Embryonic stem cells Isolation Identification |
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