Effect of Gossypol on Trypomastigotes and Amastigotes of Trypanosoma cruzi |
| |
Authors: | LEONOR E ROVAI AGUSTIN AOKI NELIA M GEREZ DE BURGOS ANTONCO BLANCO |
| |
Institution: | Cátedra de Química Biológica, C.C. 35. Suc. 16. 5016 Córdoba, Argentina;Centro de Microscopía Electrónica. Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, C.C. 35. Suc. 16. 5016 Córdoba, Argentina |
| |
Abstract: | Bloodstream Trypanosoma cruzi trypomastigotes isolated from infected mice undergo reduction of motility and structural damages after 5 to 45 min exposure to gossypol at concentrations ranging from 5 to 50 μM. When 1% serum albumin is added to the incubation medium, no alterations of parasites are observed, even with 100 μM gossypol. Intracellular T. cruzi amastigotes in infected Vero cell cultures exposed to 5 μM gossypol for 2 h do not show changes. Incubation with 5 μM gossypol for 48 h produces complete disruption of host cells; however, the amastigotes they contain show only mineor alterations. The observations indicate that, in protein-rich media, gossypol is complexed into associations which have no activity on the different forms of the T. cruzi biological cycle. |
| |
Keywords: | Chagas disease gossypol inactivation by proteins trypanocidal agent trypomastigote motility ultrastructural changes Vero cell cultures |
|
|