Comparison of the potentiometric, (31)P NMR, and zero-point titration methods of determining ionized magnesium in erythrocytes |
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Authors: | Malon Adam Wagner Barbara Bulska Ewa Maj-Zurawska Magdalena |
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Institution: | Faculty of Chemistry, Warsaw University, Pasteura 1, 02093 Warsaw, Poland. |
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Abstract: | A simple and rapid method of determining ionized magnesium in erythrocytes using a potentiometric clinical analyzer, Microlyte 6 (Kone, Finland), was investigated. The erythrocyte cell membranes were destroyed using ultrasound. The results were compared with those obtained with the (31)P nuclear magnetic resonance spectroscopy method and the zero-point titration method using atomic absorption spectrometry. The results obtained from potentiometry and from the other methods did not differ significantly (Student t test, alpha = 0.01). Total magnesium concentration was determined using atomic absorption spectrometry. |
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Keywords: | magnesium erythrocytes ion selective electrode 31P nuclear magnetic resonance spectroscopy zero-point titration |
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