任意引物PCR及其应用研究进展

任意引物PCR技术又称为随机扩增多态性DNA技术，它是在PCR技术基础上发展起来的一项分子检测技术。它具有简便、快速，一套引物可用于多个物种的分析，不需预知分析对象的核酸序列，可以显示差异表达基因等特点，已广泛应用于病原微生物的分型鉴定、物种亲源关系分析、遗传育种研究和特异表达基因的克隆与鉴定等方面。

Arbitrarily primed polymerase chain reaction and its application

s:The arbitrarily primed polymerase chain reaction(AP-PCR)fingerprinting or random amplified polymor-phic DNA(RAPD)technique is a simple and fast molecular detection method based on PCR.It requires no prior knowl edge of the molecular biology of the organisms to be investigated.A universal set of primers can be used for genomic analysis in a wide variety of species.RNA arbitrarily primed PCR can be used to identify differential ex-pression genes.The approach was widely applied in typing and identification of organisms,parentage analysis,genet-ic breeding and cloning of specific expression genes.