Antifungal mechanism of a cysteine-rich antimicrobial peptide, Ib-AMP1, from Impatiens balsamina against Candida albicans |
| |
| Authors: | Dong Gun Lee Song Yub Shin Dae-Hee Kim Moo Yeol Seo Joo Hyun Kang Younghoon Lee Kil Lyong Kim Kyung-Soo Hahm |
| |
| Affiliation: | (1) Peptide Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology, P. O. Box 115, Yusong, Taejon, 305-600, Korea;(2) Department of Chemistry, Korea Advanced Institute of Science and Technology, Yusong, Taejon, 305-701, Korea |
| |
| Abstract: | The antifungal mechanism of a 20-mer peptide, Ib-AMP1, derived from Impatiens balsamina was investigated. The oxidized (disulfide bridged) Ib-AMP1 showed a 4-fold increase in antifungal activity against Aspergillus flavus and Candida albicans than reduced (non-disulfide bridged) Ib-AMP1. Ib-AMP1 had very low activity for phospholipid disruption when compared with cecropin A(1-8)-magainin 2(1-12), a  -helical amphiphatic, antimicrobial peptide. Confocal microscopy showed that Ib-AMP1 binds on cell surface or penetrates into cell membranes. These results suggested that Ib-AMP1 may manifest its antifungal activity against Candida albicans by inhibiting a distinct cellular process rather than ion channel or pore formation in cell membrane. |
| |
| Keywords: | antifungal mechanism Candida albicans confocal microscopy Ib-AMP1 |
| 本文献已被 SpringerLink 等数据库收录！ |
|
