七种植食性金龟子雌雄成虫的鉴别方法

本研究通过对大黑鳃金龟Holotrichia oblita Faldermann、暗黑鳃金龟H.parallela Motschulsky、铅灰齿爪鳃金龟H.plumbea Hope、铜绿丽金龟Anomala corpulenta Motsch、棕色鳃金龟H.titanis Reitter、拟毛黄鳃金龟H.formosana Moser、小黄鳃金龟Metabolus flavescens Brenske7种常见植食性金龟子的雌雄腹部末端进行显微观察,建立了一种体外无损伤快速准确鉴别常见植食性金龟子雌雄的方法,即轻掰金龟子腹部末端,雌虫可见两片分离的第九腹板退化而成的瓣状褐色骨片,雄虫则无或呈倒"V"字型阳基侧突粘连骨片。该方法对实验所用的鳃金龟、丽金龟2亚科7种金龟子均适用,具有较大的广谱性,且与传统方法相比,操作简便迅速、易于掌握,准确率高。此结果对金龟子田间预测预报以及性信息素的分离研究具有重要意义。6

The method for sex discrimination of seven phytophagous scarab beetles

The abdominal ends of seven common phytophagous scarab beetles,such as Holotrichia oblita Faldermann,Holotrichia parallela Motschulsky, Holotrichia plumbea Hope, Anomala corpulenta Motsch, Holotrichia titanis Reitter,Holotrichia formosana Moser,Metabolus flavescens Brenske were observed carefully with the aid of the microscope （Olympus SZ61） and a quick and accurate method was established to distinguish the sex of beetles without causing any injury to them. As the beetles’abdominal tips were opened,two separate patches of sclerite were seen in female beetles that were absent in males. Conversely,two inversed＂V＂shape sclerite patches were observed in male beetles but not in females. Compared to the traditional methods,the method described here provided convenient,quick and accurate sexing of seven species of the Melolonthidae and Rutelidae. This method will be helpful for the forecast and control of the beetles in the field and the collection and identification of their sex pheromones.