Coenzyme A: fatty acid synthetase apoenzyme 4'-phosphopantetheine transferase in yeast |
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Authors: | K Werkmeister F Wieland E Schweizer |
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Institution: | 1. Lehrstuhl für Biochemie der Universität Erlangen-Nürnberg Egerlandstr. 7, D-8520 Erlangen W.-Germany;2. Institut für Biochemie der Universität Regensburg Universitätsstr. 31, D-8400 Regensburg W.-Germany |
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Abstract: | A mixture of two pantetheine-free mutant fatty acid synthetases was dissociated and recombined to form a hybrid apoenzyme complex. the corresponding -mutants exhibit interallelic complementation when crossed with each other and the enzyme synthesized in the resulting diploid contains pantetheine and exhibits overall fatty acid synthetase activity. Accordingly, the hybrid apoenzyme formed could be activated to holo-fatty acid synthetase when incubated with coenzyme A and a partially purified yeast cell extract. The enzyme coenzyme A: fatty acid synthetase apoenzyme 4′-phosphopantetheine transferase has thus been identified in yeast. Further studies on the mechanism of fatty acid synthetase holoenzyme formation will now be possible. |
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