Influence of hormones and growth factors on viability,DNA, and protein content of adult hepatocytes in primary culture |
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Authors: | Klaus Wirthensohn Christian A Barth |
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Institution: | 1. Forschergruppe Ern?hrung an der Medizinischen Poliklinik der Universit?t München, Pettenkoferstr. 8 a, 8000, München 2, FRG
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Abstract: | Summary The survival of adult rat hepatocytes in monolayer culture was studied in the presence of different hormones (neurotensin,
oxytocin, thyrotropin releasing hormone, luteinizing hormone releasing hormone, cholecalciferol, bradykinin, substance P,
aldosterone, melanocyte stimulating hormone, 3,3′,5-triiodo-1-thyronine, corticosterone, human growth hormone, glucagon, insulin,
progesterone, testosterone, estradiol, and dexamethasone phosphate) or growth factors (fetal bovine serum). For this purpose
trypan blue exclusion, lactate dehydrogenase, and DNA and protein content were measured at 24 and 72 h of culture. 10−7
M Dexamethasone, a mixture of eight hormones, 10% fetal bovine serum, and a combination of the latter two supplements caused
a more than 64% higher DNA content at 72 h when compared to control cultures. A striking agreement of these results with changes
of lactate dehydrogenase leakage was observed, whereas trypan blue exclusion gave erratic results. Considerable changes of
cell arrangement apparently specific for each supplement were ovserved by low magnification microscopy. It is concluded that
glucocorticoids and fetal bovine serum have an outstanding effect on cell viability and that DNA or protein content or both
are reliable indicators of cell viability in amitotic cultures. |
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Keywords: | viability hormones hepatocytes culture enzyme leakage |
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