Effects of iron,copper, zinc,calcium, and magnesium on human lymphocytes in culture |
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Authors: | U Carpentieri J Myers C W Daeschner M E Haggard |
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Institution: | (1) Departments of Pediatrics and Pathology, University of Texas Medical Branch, 77550 Galveston, TX |
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Abstract: | The effects of simultaneous changes of calcium, magnesium, iron, copper, and zinc concentrations were evaluated in normal
human T and B lymphocytes, cultured in cation-depleted media. Optimal concentrations for thymidine incorporation (TI) in both
cell populations were Fe and Zn 15 μM and Cu 5 μM; for t cells Ca 2 mM and Mg 4 mM; for B cells Ca 4 mM and Mg 6 mM. TI decreased with increasing molarity of cations and the decrease was particularly apparent with Cu. Minimal amounts of
Ca and Mg (0.5 mM) were necessary for growth, even in presence of optimal concentrations of Fe, Cu, and Zn. Fe and Cu showed synergistic stimulatory
effects at low concentrations and synergistic inhibitory effects at high concentrations. Antagonism between Fe and Zn, Cu
and Zn, and Ca and Zn was also demonstrated. CD4/CD8 increased with PHA stimulation in presence of Zn, and decreased with
ConA stimulation in presence of Zn or Fe. The results demonstrate: (1) the relationship and interdependence of Fe, Cu, and
Zn concentrations in modulating the growth of normal lymphocytes; (2) the stimulatory effects of Fe on B cells and Zn on CD8
positive cells; (3) the inhibitory effect of Cu at concentrations lower than those of Fe and Zn; (4) the requirement of Ca
and Mg in certain concentration and ratio for the action of the other cations; and (5) the Ca and Mg requirement for the growth
of B cells higher than T cells. |
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Keywords: | Lymphocytes iron copper zinc calcium magnesium |
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