A strategy for producing single-stranded DNA in the polymerase chain reaction: A direct method for genomic sequecing期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

A strategy for producing single-stranded DNA in the polymerase chain reaction: A direct method for genomic sequecing

Affiliation:	1. Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung City, Taiwan;2. Department of Civil and Environmental Engineering, University of Delaware, Newark, 19716, DE, USA;3. Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu 30013, Taiwan;1. Department of Computer Science, Cornell University, Ithaca, NY 14853, United States;2. Department of Electrical Engineering and Computer Science, MIT, Cambridge, MA 02139, United States

Abstract:	A strategy for synthesizing single-stranded DNA by the polymerase chain reaction method is explored and two protocols are developed. DNA produced with these methods can be sequenced using standard ³⁵S or ³²P dideoxy sequencing protocols. The methods allow rapid genetic screening and population genetic analysis of sequence variation.

Keywords:
本文献已被 ScienceDirect 等数据库收录！