首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Simple and efficient oligonucleotide-directed mutagenesis using one primer and circular plasmid DNA template
Institution:1. Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran;2. Department of Anaerobic Bacterial Vaccine Research and Production, Razi Vaccine and Serum Research Institute— Kerman branch, Kerman, Iran;3. Department of Immunology, Medical School of Kerman University, Kerman, Iran;1. Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Petersgasse 12/I, 8010 Graz, Austria;2. CAPEC-PROCESS Research Center, Department of Chemical and Biochemical Engineering, Technical University of Denmark, Søltofts Plads Building 229, 2800 Kgs. Lyngby, Denmark;1. OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell''Abruzzo e del Molise (IZSAM), Teramo, Italy;2. National Reference Center for Whole Genome Sequencing of microbial pathogens: database and bioinformatic analysis, Istituto Zooprofilattico Sperimentale dell''Abruzzo e del Molise, Campo Boario, 64100 Teramo, Italy;3. Univérsité de Tunis El Manar, Institut de la Recherche Vétérinaire de Tunisie (IRVT), Laboratoire de virologie, 20 Rue Djebel Lakhdar, 1006 Tunis, Tunisia;4. CRDA-Commissariats Régionaux au Developpement Agricole, Menzel Bouzelfa, Tunisia;5. CRDA, Medenine, Tunisia;6. CRDA, Gafsa, Tunisia;7. Ecole Nationale de Médecine Vétérinaire de Sidi Thabet, Tunisia
Abstract:A rapid and simple procedure for site-directed mutagenesis is described. This method uses only a single oligonucleotide primer with the double-stranded circular plasmid DNA as the template for mutagenesis. The phage T4 gene 32 product is included during primer extension in vitro to increase efficiency. Single and multiple changes as well as deletions have been obtained at an efficiency of 1–2%.
Keywords:
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号