Mercury binding proteins in liver and muscle of flat fish from the northern Tyrrhenian sea |
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| Institution: | 1. Biochemical Adaptation Laboratory, Department of Zoology, North-Eastern Hill University, Shillong 793022, India;2. Molecular Endocrinology Laboratory, Centre for Advanced Studies in Zoology, Visva-Bharati University, Santiniketan 731235, India;1. Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, Branišovská 31, 370 05 České Budějovice, Czech Republic;2. Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01 Košice, Slovak Republic;3. Life Sciences, The Natural History Museum, Cromwell Road, SW7 5BD London, England, UK;4. Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu, China;5. Uludag University, Mustafakemelpasa Vocational School, Mustafakemelpasa, Bursa, Turkey;6. Institute of Biology and Soil Science, Far Eastern Branch of Russian Academy of Sciences, Vladivostok, Russia;7. 10486-2 Hotaka-Ariake, Azumino, Nagano 399-8301, Japan |
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| Abstract: | 1. The subcellular distribution of mercury and possible presence of Hg binding proteins of low molecular weight were investigated by ultracentrifugation and gel filtration in liver and muscle of the flat fish Citharus linguatula and Lepidorhombus boscii from the northern Tyrrhenian sea, heavily contaminated by the metal.2. For both tissues, Hg contents were higher in the pellet than in the supernatant.3. In the eluate of supernatant from Sephadex G-75 of both tissues, Hg was mainly bound to high molecular weight ligands.4. Differently from the muscle eluate, that from liver also contained a consistent amount of Hg bound to low molecular weight ligands. |
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