Labeling proteins using aryl iodide acylation agents: Influence of meta vs para substitution on in vivo stability |
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| Institution: | 1. Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, 68198, USA;2. Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China;3. Department of Medicine, National Jewish Health, Denver, CO 80206, USA;4. Department of Pediatrics, National Jewish Health, Denver, CO 80206, USA;5. Department of Pediatrics, University of Colorado Health Sciences Center, Aurora, CO 80045, USA;1. Department of Molecular Pathology, Tohoku University School of Medicine, Sendai, Japan;2. Department of Gastrointestinal Surgery, Tohoku University School of Medicine, Sendai, Japan;3. Department of Digestive Tract Surgery and Transplantation Surgery, Tokyo Medical University Hachioji Medical Center, Tokyo, Japan;1. School of Life Sciences, Ulsan National Institute of Science and Technology, UNIST-gil 50, Ulju-gun, Ulsan 689-798, Republic of Korea;2. Center for Genomic Integrity, Institute for Basic Science (IBS), UNIST-gil 50, Ulju-gun, Ulsan 689-798, Republic of Korea;3. Division of Signaling and Gene Expression, La Jolla Institute for Allergy & Immunology, La Jolla, CA 92037, USA;4. Department of Pharmacology and Moores Cancer Center, University of California at San Diego, La Jolla, CA, USA;5. Sanford Consortium for Regenerative Medicine, La Jolla, CA 92037, USA |
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| Abstract: | The effect of para vs meta substitution on the biological behavior of an intact antibody and an F(ab′)2 fragment was investigated. Paired-label studies were performed using 81C6 IgG and OC 125 F(ab′)2 labeled using the N-succinimidyl esters of both p-125I]- and m-131I]iodobenzoate as well as with the potential catabolites, p-125I]- and m-131I]iodobenzoic acid. In all 3 studies, up to 55% lower uptake of 131I in thyroid and stomach was observed, suggesting that the m-substituted species were more inert to dehalogenation in vivo. |
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