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Improved methods of estimating root length using a photocopier,a light box and a bar code reader
Authors:P. R. Collins  P. J. Gregory  H. R. Rowse  A. Morgan  B. Lancashire
Affiliation:(1) Department of Soil Science, University of Reading, London Road, RE1 5AQ Reading, UK;(2) National Vegetable Research Station, CV35 9EF Wellesbourne Warwick, UK
Abstract:Photocopying was found to be a rapid method of making a permanent record of a root sample. The method used produced a copy with white roots against a black background. Manual estimates of root length were made from photocopies using a light box. The number of intersections visible when laid over a copy of a white on black regular square grid was counted. Automated estimates of root length were made by scanning a photocopy with a bar code reader in place of a pen in a computer-driven graph plotter. Roots >0.2 mm diameter were resolved with precision and speed.
Keywords:root length
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