Cloning and expression of 5, 10-Methylenetetrahydrofolate reductase (MTHFR) gene |
| |
| Authors: | Guanghui Chen Chenhui Zhang Xiaoyu Zhang Yanqing Zhu Jian Tang Zhaoheng Meng Zhu Li |
| |
| Institution: | 1. Institute of Cardiovascular Research, Beijing Medical University, 100083, Beijing, China
2. Department of Health Care Epidemiology, Beijing Medical University, 100083, Beijing, China
|
| |
| Abstract: | Hyperhomocysteinemia is an independent risk factor of cardiovascular diseases and birth defects. One of the important factors causing hyperhomocysteinemia is decrease of 5,10-Methylenetetrahydrofdate reductase. Human and rat MTHFR cDNAs with RT-PCR were isolated, a prokaryodytic expression vector containing human MTHFR cDNA was constructed, and human MTHFR protein was expressed inE. coli. It was also found that the expression of rat MTHFR could be promoted by IL-1 and homocysteine. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
