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Isolation of monomeric photosystem II that retains the subunit PsbS |
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| Authors: | Patrycja Haniewicz Daniele De Sanctis Claudia Büchel Wolfgang P Schröder Maria Cecilia Loi Thomas Kieselbach Matthias Bochtler Dario Piano |
| Institution: | 1. International Institute of Molecular and Cell Biology, Ul. Ks. Trojdena 4, 02109, Warsaw, Poland 2. European Synchrotron Radiation Facility, Rue Jules Horowitz 6, 38000, Grenoble, France 3. Institute of Molecular Biosciences, University of Frankfurt, Max von Laue Stra?e 9, 60438, Frankfurt am Main, Germany 4. Ume? Plant Science Center and Institute of Chemistry, Linnaeus v?g 10, 90187, Ume?, Sweden 5. Department of Life and Environmental Sciences, University of Cagliari, V.le S. Ingnazio da Laconi 13, 09123, Cagliari, Italy 6. Department of Bioinformatics, Institute of Biochemistry and Biophysics, Pawinskiego 5a, 02-106, Warsaw, Poland |
| Abstract: | Photosystem II has been purified from a transplastomic strain of Nicotiana tabacum according to two different protocols. Using the procedure described in Piano et al. (Photosynth Res 106:221–226, 2010) it was possible to isolate highly active PSII composed of monomers and dimers but depleted in their PsbS protein content. A “milder” procedure than the protocol reported by Fey et al. (Biochim Biophys Acta 1777:1501–1509, 2008) led to almost exclusively monomeric PSII complexes which in part still bind the PsbS protein. This finding might support a role for PSII monomers in higher plants. |
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