Microwave treatment induced mutations and altered gene expression in Vigna aconitifolia |
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Authors: | R. K. Jangid R. Sharma Y. Sudarsan S. Eapen G. Singh A. K. Purohit |
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Affiliation: | 1.Plant Biotechnology Centre,Rajasthan Agricultural University,Bikaner,India;2.ASC-DEC,Rajasthan Agricultural University,Bikaner,India;3.NABTD,Bhabha Atomic Research Centre,Trombay, Mumbai,India |
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Abstract: | Primary leaf explants of aseptically grown seedlings of moth bean [Vigna aconitifolia (Jacq.) Marechal] immersed in water or not were treated in microwave oven (2450 MHz, 800 W cm−2) for 1, 3, 5 and 7 s before culturing. Callusing and shoot emergence from these explants were enhanced up to microwave exposure lasted 5 s while longer treatment of water-immersed explants delayed callusing. One polypeptide (26.6 kD) was up regulated in the callus derived from microwave treatment in water-immersed explants. RAPD analysis detected alteration in DNA sequences due to microwave treatment in water-immersed explants for 7 s. The frequency of mutation was 1.6 % (4 bands out of 248) over all the cultures analyzed and the same was 13 % (4 bands out of 31), if amplicons generated at 7 s treatment alone were considered. |
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