Aggregation-induced alterations in fibroblast morphology |
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Authors: | Dr Bernadette Van der Schueren Jean-Jacques Cassiman Herman Van Den Berghe |
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Institution: | (1) Division of Human Genetics, Department of Human Biology, Leuven, Belgium;(2) Lab. Electronenmicroscopie, Centrum voor Menselijke Erfelijkheid, Minderbroederstraat 12, B-3000 Leuven, Belgium |
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Abstract: | Summary The different stages during aggregation of diploid human skin fibroblasts have been examined by transmission and scanning electron microscopy. As a result of aggregation, fibroblasts form a complex tissue configuration. Numerous intercellular junctions can be observed, while the cells remain polygonal and do not develop an organised intracellular cytoskeleton. Cell division occurs only rarely. After aggregation, signs of progressive auto-digestion develop.Adhesion to a substrate results in outgrowth of the cells and monolayer formation, even when extensive cell damage had occurred. The morphology of fibroblasts in aggregates and in the monolayers, from which they were derived, is compared and the contribution of the aggregate system to the study of fibroblast behavior is discussed.J.J. Cassiman is Aangesteld Navorser van The National Foundation for Scientific Research, Belgium |
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Keywords: | Fibroblast Human Transmission electron microscopy Scanning electron microscopy Aggregation |
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