Transformation of folate-consuming Lactobacillus gasseri into a folate producer |
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Authors: | Wegkamp Arno Starrenburg Marjo de Vos Willem M Hugenholtz Jeroen Sybesma Wilbert |
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Affiliation: | Wageningen Centre for Food Sciences and NIZO food research, Ede, The Netherlands. |
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Abstract: | Five genes essential for folate biosynthesis in Lactococcus lactis were cloned on a broad-host-range lactococcal vector and were transferred to the folate auxotroph Lactobacillus gasseri. As a result L. gasseri changed from a folate consumer to a folate producer. This principle can be used to increase folate levels in many fermented food products. |
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