Heat stress screening of peanut (<Emphasis Type="Italic">Arachis hypogaea</Emphasis> L.) seedlings for acquired thermotolerance |
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Authors: | Michael?Gomez?Selvaraj Gloria?Burow John?J?Burke Vikas?Belamkar Naveen?Puppala Email author" target="_blank">Mark?D?BurowEmail author |
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Institution: | (1) Texas AgriLife Research, Texas A&M System, 1102 East FM 1294, Lubbock, TX 79403, USA;(2) USDA-ARS Plant Stress and Germplasm Development Unit, 3810 4th Street, Lubbock, TX 79415, USA;(3) Department of Plant and Soil Science, Texas Tech University, Lubbock, TX 79409, USA;(4) Agricultural Sciences Center, New Mexico State University, Clovis, NM 88001, USA; |
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Abstract: | Heat can be one of the major abiotic stresses that adversely affect crop production worldwide at different stages of development.
As field screening for heat tolerance can be inconsistent and seasonally-limited, it is important to develop a reliable protocol
under controlled conditions that allows simultaneous screening of multiple genotypes. The objective of this research was to
develop a straightforward laboratory protocol using acquired thermotolerance (ATT) in peanut seedlings as a measure of one
mechanism of heat stress tolerance. Sixteen genotypes, including selected accessions of the US peanut minicore collection
along with standard checks, were evaluated for acquired themotolerance in two independent experiments. A change in the temperature
sensitivity of chlorophyll accumulation was used as an indicator of acquired thermotolerance. Pre-incubation at 38°C for 4 h
before the 30-min 50°C challenge triggered the acquired thermotolerance system of the leaf disks, resulting in chlorophyll
accumulation upon exposure to light. There was considerable variation among genotypes for ATT in both experiments. Genotypic
ranking for mean ATT values were highly correlated (0.949) in both experiments. The effect of seed weight on ATT was not significant.
This method is relatively simple and inexpensive and can be used to screen a large number of genotypes. |
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