Microspore culture protocol for Indonesian Brassica oleracea |
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Authors: | Budi Winarto Jaime A. Teixeira da Silva |
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Affiliation: | (1) Indonesian Center of Horticultural Research and Development, Jl. Ragunan No 29A, Pasar Minggu, Jakarta Selatan, Indonesia;(2) Faculty of Agriculture and Graduate School, Kagawa University, Miki-cho, Kagawa 761-0795, Japan |
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Abstract: | A microspore culture protocol for Brassica oleracea of Indonesian origin (cv. ‘Kemeh’) has been successfully established. A high number of embryos formed with high microspore density i.e. 15 × 104 cells/ml. Embryo formation was improved by using flower buds (4.5–4.6 mm in length) as explants, a temperature treatment at 30.5°C for 48 h and then transfer to 25°C continuously until embryos formed. A total of 295 embryos were obtained from 189 buds, 30% of which were abnormal (i.e. with an abnormal cotyledon or lacking hypocotyls). All normal embryos that grew and survived, 165 in total, were successfully transferred to soil and grew well in plastic bags (15 cm in diameter) containing a mixture of burned-rice husk and organic manure (1:1, v/v). |
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