Glutamate as a differential nitrogen source for the characterization of acetylene-reducing Rhizobium strains |
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Authors: | T Kaneshiro MA Kurtzman |
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Institution: | Northern Regional Research Center, Agricultural Research, Science and Education Administration, US Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604, USA |
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Abstract: | A majority (36 of 44) of Rhizobium japonicum strains tested reduced acetylene asymbiotically when grown on an agar medium containing 0.1% (w/v) L-glutamate as a sole nitrogen (N) source. Glutamate as N source led to pinpoint colonies and uniform glutamine synthetase activity of three selected, slow-growing acetylene-reducing strains ( R. japonicum L-259 and 110 and a cowpea-type Rhizobium 32H1). The three test strains were characterized further by antibiotic resistance, colony type, cellular morphology, and differential growth on different N sources. The evidence suggests that, in an agar medium, glutamate creates a growth condition leading to acetylene reduction activity, pinpoint colonies and pleomorphism. |
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