Utilization of exogenous sugars by excised maize embryos in culture |
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Authors: | Kornélia Burghardtová J Tupý |
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Institution: | (1) Institute of Botany, Slovak Academy of Sciences, Bratislava;(2) Present address: Research Institute of Petroleum and Hydrocarbon Gases, 82311 Brati-slava-Vlčie hrdlo, Czechoslovakia;(3) Institute of Experimental Botany, Czechoslovak Academy of Sciences, Flemingovo nám. 2, 16000 Praha 6, Czechoslovakia |
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Abstract: | Sucrose was markedly superior to fructose and glucose in promoting growth of plantlets from immature maize embryos. The elongation
of roots is shown to be more sucrose dependent than that of shoots. On the other hand, the exogenous sucrose was less effective
than fructose as substrate for carbohydrate catabolism and for the synthesis of alcohol-insoluble compounds at the beginning
of embryo cultivation. The absorbed fructose was found to be rapidly converted to sucrose and the level of endogenous sucrose
derived from sugar supplied to the medium was higher in fructosethan in sucrose-fed embryos. The preferential utilization
of fructose over sucrose, however, declined with the progress of germination which may be related to the decrease in proportion
of scutellum in total mass and physiological activity of the embryo. |
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Keywords: | |
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